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Journal of Integrative Medicine: Volume 11, 2013   Issue 4,  Pages: 253-261

DOI: 10.3736/jintegrmed2013025
Research Article
Pharmacological evaluation of Musa seminifera Lour. fruit
1. Sanjib Saha (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh E-mail: sanjibsaha1991@yahoo.com)
2. Faroque Hossain (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
3. Md. Anisuzzman (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )
4. Md. Khirul Islam (Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh )

OBJECTIVE: To study potential antioxidant, analgesic, antidiarrheal, and antibacterial activities of the ethanol extract of Musa seminifera Lour. fruit in different established in vivo and in vitro experimental models.

METHODS: In vitro antioxidant activity was studied in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Phenolic content was determined using Folin-Ciocalteu’s reagent. Reducing ability was evaluated by ferric reducing power assay. Peripherally and centrally acting analgesic activity was studied in three different in vivo models, namely, acetic acid-induced writhing, hot-plate test, and tail-flick test in Swiss albino mice. In vivo antidiarrheal activity was evaluated in castor oil- and magnesium sulfate-induced diarrhea in mice. Gastrointestinal motility test was also carried out in mice. All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight. Antibacterial activity was assessed by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains. Acute toxicity test was conducted to assess the safe doses of the extract.

RESULTS: The extract showed 50% inhibitory concentration value of 12.65 μg/mL in DPPH radical-scavenging assay. Phenolic content was found to be 589.83 mg gallic acid equivalent per 100 g of dried fruits extract. Reducing power was in a concentration-dependent manner, and strongly comparable with the standard ascorbic acid. The extract demonstrated significant inhibition of writhing in acetic acid-induced writhing test at both dose levels (P<0.01). The extract also raised pain threshold in both hot-plate and tail-flick test in a dose-dependent manner, and the results were statistically significant (P<0.01). The extract significantly (P<0.01) increased latent period, and decreased defecation in both castor oil- and magnesium sulfate-induced diarrhea. The extract also decreased gastrointestinal motility in mice. In disk diffusion assay, the extract showed potential antibacterial activity against all the tested bacterial strains.

CONCLUSION: The results suggest that the ethanol extract of M. seminifera fruit has potential antioxidant, analgesic, antidiarrheal, and antibacterial activities.

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Saha S, Hossain F, Anisuzzman M, Islam MK. Pharmacological evaluation of Musa seminifera Lour. fruit. J Integr Med. 2013; 11(4): 253-261.
1Samuelson AD. Medicinal values of plantain. 3rd ed[M]. Oxford and Edinburgh: Blackwell Scientific Publication, 1980. 30-37.  
2Tapsell LC, Hemphill I, Cobiac L, Patch CS, Sullivan DR, Fenech M, Roodenrys S, Keogh JB, Clifton PM, Williams PG, Fazio VA, Inge KE. Health benefits of herbs and spices: The past, the present, the future[J].Med J Aust, 2006, 185(4 Suppl): S4-S24.  
3Partha P, Hossain ABME. Ethanobotanical investigation into the Mandi ethnic community in Bangladesh[J].Bangladesh J Plant Taxon, 2007, 14(2): 129-145.  
4Ghani A. Medicinal plants of Bangladesh: chemical constituents and uses. 2nd ed[M]. Dhaka: The Asiatic Society of Bangladesh, 2003. 315.  
5Khare CP. Indian medicinal plants: An illustrated dictionary[M]. New York: Springer Science+Business Media, LLC, 2007. 426.  
6Goel RK, Sairam K. Anti-ulcer drugs from indigenous sources with emphasis on Musa sapientum, Tamrabhasma, Asparagus racemosus and Zingiber officinale[J].Indian J Pharmacol, 2002, 34(2): 100-110.  
7Zafar IM, Saleha A, Hoque MME, Sohel RM. Antimicrobial and cytotoxic properties of different extracts of Musa sapientum L. subsp. sylvestris[J].Indian J Exp Biol, 2011, 2(8): 62-65.  
8Agarwal PK, Singh A, Gaurav K, Goel S, Khanna HD, Goel RK. Evaluation of wound healing activity of extracts of plantain banana (Musa sapientum var. paradisiaca) in rats[J].Indian J Exp Biol, 2009, 47: 322-340.  
9Tewtrakul S, Itharat A, Thammaratwasik P, Ooraikul B. Anti-allergic and anti-microbial activities of some Thai crops[J].Songklanakarin J Sci Technol, 2008, 30(4): 467-473.  
10Mallick C, Chatterjee K, Guhabiswas M, Ghosh D. Antihyperglycemic effects of separate and composite extract of root of Musa paradisiaca and leaf of Coccinia indica in streptozotocin-induced diabetic male albino rat[J].Afr J Tradit Complement Altern Med, 2007, 4(3): 362-371.  
11Waalkes TP, Sjoerdsma A, Creveling CR, Weissbach H, Udenfriend S. Serotonin, norepinephrine, and related compounds in Bananas[J].Science, 1958, 127(3299): 648-650.  
12Shanmugavelu KG, Rangaswami G. Tryptophan and indole compounds in banana ovaries[J].Nature, 1962, 194: 775-776.  
13Lewis DA, Shaw GP. A natural flavonoid and synthetic analogues protect the gastric mucosa from aspirin-induced erosions[J].J Nutr Biochem, 2001, 12(2): 95-100.  
14Lewis DA, Fields WN, Shaw GP. A natural flavonoid present in unripe plantain banana pulp (Musa sapientum L. var. paradisiaca) protects the gastric mucosa from aspirin-induced erosions[J].J Ethnopharmacol, 1999, 65(3): 283-288.  
15Ghoshal S. Steryl glycosides and acyl steryl glycosides from Musa paradisiaca[J].Phytochemistry, 1985, 24(8): 1807-1810.  
16Mokbel MS, Hashinaga F. Antibacterial and antioxidant activities of banana (Musa, AAA cv. Cavendish) fruits peel[J].Am J Biochem Biotechnol, 2005, 1(3): 125-131.  
17Sadhu SK, Okuyama E, Fujimoto H, Ishibashi M. Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities[J].Chem Pharm Bull, 2003, 51(5): 595-598.  
18Sharma OP, Bhat TK. DPPH antioxidant assay revisited[J].Food Chem, 2009, 113(4): 1202-1205.  
19Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables[J].J Univ Chem Technol Metallurgy, 2005, 40(3): 255-260.  
20Talukder C, Saha S, Adhikari S, Mondal HK, Islam MK, Anisuzzman M. Evaluation of antioxidant, analgesic and antidiarrhoeal activity of Flacourtia jangomas (Lour.) Raeusch. leaves[J].Pharmacologyonline, 2012, 3: 20-28.  
21Oyaizu M. Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine[J].Jpn J Nutr, 1986, 44: 307-315.  
22Lorke D. A new approach to practical acute toxicity testing[J].Arch Toxicol, 1983, 54(4): 275-287.  
23Koster R, Anderson M, De Beer EJ. Acetic acid for analgesics screening[J].Fed Proc, 1959, 18: 412-417.  
24Saha S, Islam MK, Anisuzzman M, Hasan MM, Hossain F, Talukder C. Evaluation of antioxidant, analgesic and antidiarrhoeal activity of Phoenix paludosa Roxb. leaves[J].Int J Basic Med Sci Pharm, 2012, 2: 46-52.  
25Karmakar UK, Sultana R, Biswas NN. Antioxidant, analgesic and cytotoxic activities of Mimusops elengi Linn. leaves[J].Int J Pharm Sci Res, 2011, 2(11): 2791-2797.  
26Eddy NB, Leimback D. Synthetic analgesics. II. Dithienyl-butenyl and dithienybutylamines[J].J Pharmacol Exp Ther, 1953, 107(3): 385-393.  
27Aydin S, Demir T, Oztürk Y, Ba?er KH. Analgesic activity of Nepeta italica L[J].Phytother Res, 1999, 13(1): 20-23.  
28Abdullahi AL, Agho MO, Amos S, Gamaniel KS, Wambebe C. Antidiarrhoeal activity of the aqueous extract of Terminalia avicennoides roots[J].Phytother Res, 2001, 15(5): 431-434.  
29Doherty NS. Inhibition of arachidonic acid release as the mechanism by which glucocorticoids inhibit endotoxin-induced diarrhoea[J].Br J Pharmacol, 1981, 73(2): 549-554.  
30Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method[J].Am J Clin Pathol, 1966, 45(4): 493-496.  
31Saha S, Anisuzzman M, Islam MK, Mondal H, Talukder C. Antibacterial and cytotoxic potential of Dalbergia spinosa Roxb. leaves[J].Int J Pharm Sci Res, 2013, 4(1): 512-515.  
32Paix?o N, Perestrelo R, Marques JC, Camara JS. Relationship between antioxidant capacity and total phenolic content of red, rosé and white wines[J].Food Chem, 2007, 105(1): 204-214.  
33Razali N, Razab R, Junit SM, Aziz AA. Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale)[J].Food Chem, 2008, 111(1): 38-44.  
34Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions[J].Food Chem, 2003, 83(4): 547-550.  
35Li HY, Hao ZB, Wang XL, Huang L, Li JP. Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance[J].Bioresour Technol, 2009, 100(2): 970-974.  
36Ribeiro SMR, Barbosa LCA, Queiroz J, Knodler HM, Schieber A. Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties[J].Food Chem, 2008, 110(3): 620-626.  
37Headley PM, O’Shaughnessy CT. Evidence for opiate and dopamine interaction in striatum[J].Br J Pharmacol, 1985, 86: 700.  
38Wigdor S, Wilcox GL. Central and systemic morphine-induced antinociception in mice: Contribution of descending serotonergic and noradrenergic pathways[J].J Pharmacol Exp Ther, 1987, 242(1): 90-95.  
39Pierce NF, Carpenter CC Jr, Elliott HL, Greenough WB 3rd. Effects of prostaglandins, theophylline, and cholera exotoxin upon transmucosal water and electrolyte movement in the canine jejunum[J].Gastroenterology, 1971, 60(1): 22-32.  
40Zavala MA, Pérez S, Pérez C, Vargas R, Pérez RM. Antidiarrhoeal activity of Waltheria americana, Commelina coelestis and Alternanthera repens[J].J Ethnopharmacol, 1998, 61(1): 41-47.  
41Moreno S, Scheyer T, Romano CS, Vojnov AA. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition[J].Free Radic Res, 2006, 40(2): 223-231.  
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