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Journal of Integrative Medicine: Volume 17, 2019   Issue 2,  Pages: 141-146

DOI: 10.1016/j.joim.2019.01.004
Original Experimental Research
Extract of Emblica officinalis enhances the growth of human keratinocytes in culture
1. Yoshimi Yamakami (Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan )
2. Kyoko Morino (Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan )
3. Yuki Takauji (Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan )
4. Ryuichiro Kasukabe (Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan )
5. Kensuke Miki (Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan )
6. Mohammad Nazir Hossain (3.Department of Biochemistry, Primeasia University, Dhaka 1213, Bangladesh )
7. Dai Ayusawa (Ichiban Life Corporation, Naka-ku, Yokohama 231-0048, Japan )
8. Michihiko Fujii (Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan E-mail: mifuji@yokohama-cu.ac.jp)

ABSTRACT

Objective: Keratinocytes are the predominant cell type in the epidermis and play key roles in epidermal function. Thus, identification of the compounds that regulate the growth of keratinocytes is of importance. Here the authors searched for such compounds from the herbs used in traditional medicine Ayurveda.

Methods: Human keratinocytes were cultured in the presence or absence of the herbal extracts for 2 weeks; the effect of the extracts on cell growth was determined by staining the cells with Coomassie brilliant blue. To detect the compounds that regulate the growth of keratinocytes, the herbal extracts were subjected to high-performance liquid chromatography (HPLC).

Results: It was found that the extract of Emblica officinalis enhanced the growth of keratinocytes in culture. Further, the authors fractionated the extract of E. officinalis using HPLC and identified the fractions responsible for the enhanced growth of keratinocytes. 
Conclusion: The extract of E. officinalis enhanced the growth of human keratinocytes in culture. E. officinalis contains the compounds that would be beneficial for human skin health because enhanced growth of keratinocytes would promote wound healing.
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Please cite this article as:
Yoshimi Yamakami, Kyoko Morino, Yuki Takauji, Ryuichiro Kasukabe, Kensuke Miki, Mohammad Nazir Hossain, Dai Ayusawa, Michihiko Fujii. Extract of Emblica officinalis enhances the growth of human keratinocytes in culture. J Integr Med. 2019; 17(2): 141-146.
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